Documentation

About the SNV-MWAS catalog

Gut microbes harbour millions of single nucleotide variants (SNVs). This resource catalogues associations between core-genome common SNVs in the human gut microbiome and host phenotypes / environmental exposures from a phenome-scale metagenome-wide association study (MWAS).

Across 6 cohorts and 10,781 host individuals, the study genotyped 12,761,497 microbial SNVs in 433 gut species against 244 phenotypes. Meta-analysis yielded 49,346 study-wide significant associations (Pmeta < 5.42×10⁻¹¹), linking 39,663 SNVs from 101 species to 69 phenotypes and exposures.

What is in the online catalog?

The browsable / downloadable table contains 1,389,120 association rows spanning cohort-level (DMP) and meta-analysis results, with allele frequencies, effect sizes, p-values, gene products, UniRef/Pfam annotations, SNV consequence classes and significance flags.

Overview KPIs describe the full study scale; charts on the Overview page summarise the released association catalog that Explore queries.

What is a row?

Each row is one SNV–phenotype association within a study stratum (e.g. Meta-analysis or DMP): the microbial SNV (species + genomic position + alleles), the host trait, sample size, effect estimate, and annotation of the gene (if coding) that contains the SNV.

  • StudyWideSig=TRUE — passes the study-wide significance cutoff for that analysis.
  • Meta-analysis — combined evidence across cohorts; default Explore view.
  • Missense / Synonymous / Non-coding — predicted SNV consequence.

How associations were derived

  1. Genotype common core-genome SNVs in gut microbial species from host metagenomes (UHGG references).
  2. Test associations with host phenotypes and exposures within cohorts; meta-analyse shared traits.
  3. Apply species-wide and study-wide significance thresholds; annotate variant consequences and gene products.
  4. Release the association catalog with interactive Explore and bulk downloads.

Using this website

  • Overview — interactive statistics; click phenotype or species bars to open Explore with filters applied.
  • Explore — unified search, filters, phenotype/species info cards, column selector and CSV export.
  • Download — complete TSV plus genomes, GFF3, UniProt and CDS resources.

How to cite

If you use SNV-MWAS data, please cite:

Daoming Wang, et al. Microbiome-wide PheWAS links gut microbial SNVs to human health and exposures, 25 September 2024, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-5063726/v1]

Contact

Fu Lab · Groningen Microbiome Hub · University Medical Center Groningen